DNA OLIGOS

RNA OLIGOS

PCR PROBES

OLIGONUCLEOTIDES SERVICES

PCR PROBES

High Quality

  • Stringent quality control (online Trityl monitoring and MALDI-TOF MS or analytical PAGE)
  • Collaboration with a pioneer with a successful track record of over 30 years

Service Portfolio

  • Wide variety of fluorophores – quencher combinations
  • EN ISO 13485:2016 certified production process

Cost Effective

  • Competitive pricing
  • High guaranteed yields

Technical Support

  • Our scientists are happy to support you
  • Hints & Tips of Handling and Storage Link

How to order:

Probe Type  Advantages

 

  Disadvantages  Applications
Dual-Labelled Probes Most popular PCR chemistry relying on the activity of Taq polymerase. Major benefits are the increased sensitivity & specificity compared to dye-based qPCR/ dPCR.

 

 Well suited for simple experiments but might not work for more complex targets. Chemistry of choice for most quantification as well as for multiplexing applications. Widely used in academic, food, environmental and medical research.
Double-Quenched Probes Marked decrease in background fluorescence compared to identical single-quenched probes. More expensive than conventional single-quenched probes.

 

 Especially suitable for demanding qPCR applications that require greater flexibility in sequence selection without sacrificing the sensitivity of the highest performing probe designs.

 
Molecular Beacons The stem probe structure of a molecular beacon makes it better able to discriminate single base-pair mismatches because the hairpin makes mismatched hybrids thermally less table than hybrids.

 

 The main disadvantage associated with molecular beacons is the accurate design of the hybridization probe. Optional design of the molecular beacon stem annealing strength is crucial. Molecular beacons have become popular for standard analyses such as quantification of DNA and RNA. Molecular beacons can be used in non-PCR implication assays.
MGB Probes Higher target binding selectivity. Less background fluorescence. Higher quality of the prove due to shorter length (between 13 and 18 nucleotides). Very robust technology.

 

 Freedom to operate in diagnostic procedure is limited due to various patents in the field. Incorporation of minor groove binders results in higher accuracy and confidence for difficult targets. Typical applications are multiplex PCR systems and low copy assays.
LNA Probes Enhanced binding affinity of LNA Tm can be fine-tuned according to the needs of a desire’s oligonucleotide. Freedom to operate in diagnostic procedures is limited due to various patents in the field. Essentially same uses as MGB probes. Especially useful for single nucleotide polymorphism (SNP) testing since LNA can be incorporated at exact position.

 
Propynyl-dC,

-du Modified Probes

 Enhance Tm selectively with the substitution of C and T. Smaller increase of Tm than LNA or MGB. Freedom to operate in diagnostic procedures is limited due to various patents in the field.

 

 Multiplex PCR Systems.

We deliver qPCR probes of the highest quality. The majority of the qPCR probes are purified by HPLC whereas a few require PAGE purification in order to meet the stringent QC specifications. If your dye/quencher combination is not compatible with HPLC just use PAGE.

Contact Us

Medical Innovation Ventures Sdn. Bhd (Mediven)

Plot 88F, Lintang Bayan Lepas 10,
Bayan Lepas Industrial Park,
Bayan Lepas Free Industrial Zone Phase 4,
11900 Bayan Lepas, Pulau Pinang, Malaysia.

Tel: +604-3052730 / Fax: +604-3052730

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